원문정보
초록
영어
Pretreatment of the Undaria pinnatifida prior to saccharification must be performed to increase the yield of reducing sugar. This study was carried out to optimize pretreatment conditions for bioethanol production. Pretreatment by thermal acid hydrolysis was performed at different sulfuric acid concentration (0.2-1% (w/v) H2SO4), treatment time (15-60 min) and solid contents of slurry (10-20% (w/v)) using Undaria pinnatifida. The effect of three variables was analyzed by using a Response-Surface Methodology(RSM). Optimal pretreatment conditions were determined as 0.7% sulfuric acid and 18% slurry at 12 1℃ for 60 min. A maximum reducing sugar of 19.2 g/L and viscosity of 33.19 cP were obtained. The pretreated seaweed was further saccharified by the treatment implementing isolated marine bacteria and commercial enzymes after neutralization by 5N NaOH. The enzyme treatment was performed by adding 1% (v/v) commercial enzymes, and then incubated at 55℃ with 120 rpm in water-bath for 4 days. Ten percent (v/v, 0.468 g dcw/L) isolated marine bacteria was added and the mixture was incubated at 30℃ with 200 rpm for 8 days.
An increase of reducing sugar and decrease of viscosity were observed.