원문정보
초록
영어
Once released into soil, organic pollutants undergo sorption and sequestration which results in reduced availability and toxicity of the pollutants [1‐2]. A novel reporter bacterial strain was constructed to quantitatively determine and visualize such behavior of the chemicals in the presence of various model solids. The reporter bacterium harbors a cell-killing gef gene and is to die and lose its fluorescence when the strain starts to biodegrade phenanthrene [3]. Four types of model solids (i.e., nonporous/porous hydrophilic beads and nonporous/porous hydrophobic beads) were chosen to represent two important soil parameters organic carbon content and porosity mostly affecting sorption of the chemical.
Phenanthrene freshly spiked into the model solids showed the different biodegradability depending on the properties of the model solids. Also, the reporter bacterium showed quantitative and linear relationships between the amounts of phenanthrene biodegraded and the extents of cell death. The reporter bacterial strain developed in the study may find a useful means to determine both bioavailable fraction and toxicity of phenanthrene in soil.