earticle

영어

Redox biocatalysis comprises a fast growing arsenal of synthetically and industrially relevant reactions with high selectivity and interesting value added products. For preparative purposes, the biocatalysts are mostly (living) microbial cells including complex, often multicomponent oxygenases for meeting basic requirements for cofactor regeneration and enzyme stability. In addition, living cells synthesize and regenerate the catalytically active oxygenases, e.g. after reactive inactivation during catalysis. Engineering living microbial cells with the objectives turnover number, total turnover number (specific activity and volumetric productivity) poses significant challenges, often integrating different levels like the enzyme, the microbial cell, the reaction and the overall process. Systems biotechnology offers a previously not available experimental and theoretical frame of methodologies and concepts to address these challenges. The focus on a certain product is the main difference of systems biotechnology in comparison to systems biology. Ultimately, understanding regulatory functions of physiology and metabolism on a genome scale for biocatalytic product formation will transform applications of cellular m icrobial biocatalysts from individual and case specific R&D endeavors to rational, modular, and concept based design approaches. This will be exemplified by studying the link of metabolic capacity and asymmetric epoxidation of styrene to (S)‐styrene oxide in recombinant E. coli and Pseudomonas sp. using flux balance analysis and metabolic flux analysis. Process data will also be evaluated with respect to environmental impact and economic significance.