원문정보
초록
영어
The objective of this study was to design nanoscale biocatalyst system by utilizing the membrane as nanoporous media. The nanostructure was modified by two step methods: simple adsorption and continue with pressure driven filtration. Two types of polymeric membranes mixed cellulose ester (MCE) and polyethersulfone (PES) were used as matrices for immobilization of lipase from Pseudomonas fluorescens.
The lipase solution was allowed to permeate through the membrane and lipase molecule was then adsorbed onto inner wall of pores. The porosity and membrane matrices influenced enzyme loading. The best result for enzyme loading in membrane matric was 3,75 g/m2 using PES membrane with incubation time of 18 hours. PES membrane was selected for further continuous transesterification studies. We evaluated the transesterification activity by converting triolein and methanol to methyl oleate and glycerol. The reaction was carried out in situ within the pores of membrane matric, so that its pores behaved as nanoreactor during formation of product. The triolein conversion using this kind of nanoreactor was about 80% with 30 minute of residence time. The productivity of immobilized lipase within the pores were 45 fold higher than that of native free lipase.