원문정보
초록
영어
Nanotechnology is currently receiving considerable attention in various fields of biotechnology. The uptake of nanoparticles by cells for labeling and tracking is a critical process for many biomedical therapeutic applications. However, nanoparticle labeling of porcine hematopoietic cells has not been demonstrated so far. In the present study, silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (SR-RITC) were used to investigate the uptake of nanoparticles by porcine hematopoietic cells. Flow cytometric and confocal microscopic analyses reveled that the cells were efficiently internalized by the silica-coated nanoparticles. Furthermore, biocompatibility tests demonstrated that the SR nanoparticles were not cytotoxic, and they had no impact on proliferation. Our study demonstrates that silica-coated nanoparticles are taken up very rapidly and with high efficiency into porcine hematopoietic cells, with no apparent deleterious effects. Therefore, silica-coated nanoparticles appear to be a promising tool for tracking porcine hematopoietic cells.
목차
INTRODUCTION
MATERIALS AND METHODS
Nanoparticles
Porcine Hematopoietic Cells
Flow Cytometry Analysis
Cell Viability and Proliferation Assay
Cellular Distribution of SR Nanoparticles
Statistics Analysis
RESULTS
Determination of SR Nanoparticles Uptake by Porcine Hermatopoietic Cells
Cytotoxicity and Effects of Nanoparticles
Intracellular Uptake of Nanoparticles
DISCUSSION
REFERENCES