원문정보
Development of Heterologous ELISA System for Diagnosis of Pregnancy in Swine
초록
영어
Early diagnoses of pregnancy for animal such as swine and bovine is extremely important to increase income of a farmhouse and for the management of farm. For the development of immunoasaay system of pregnancy in swine, we report a competitive heterologous enzyme linked immunosorbent assay (ELISA) for the direct measurement of oestrone sulfate (E1S) in diluted urine using anti-E1G (glucuronide) monoclonal antibody which cross react with E1S. The principle of assay was based on the typical solid-phase competitive ELISA methods using E1G-HRP (horseradish peroxidase) as a tracer and E1S for standard. The method had a reasonable sensitivity for the detection of E1S with 0.15 ng/ml as a detection limit. The intra-assay and inter-assay precisions were raging coefficient of from 8.50~9.67% and 8.50~9.87%, respectively, which were quite acceptable. In a field trial with a group 37 sows (18 non-pregnancy and 19 pregnancy sows) after day 29~30 post service, the concentration of E1S were determined to be below 30 ng/ml in all non-pregnancy group and over 48 ng/ml in pregnancy group except one sample. The method described here, heterologous ELISA for the measurement of E1S in urine is good enough for monitoring the early pregnancy test of swine.
목차
서론
재료 및 방법
재료
돼지뇨 시료 확보
EIG에 대한 항체
EIG-HRP Conjugate 합성
EIG-HRP 및 항체의 적정 농도 결정
항체의 선절
EIS에 대한 표준곡선의 작성
반복성 및 회수율 조사
돼지 뇨 중의 EIS 함량 측정
결과
EIG-HRP Conjugate의 분리 및 농도 결정
ELJSA 표준곡선 작성을 위한 항체의 적정 농도 결정
EIS 측정을 위한 표준곡선 작성
반복성 및 회수율(Recovery) 조사
실제뇨를 이용한 임신과 비임신뇨 측정
고찰
인용문헌