earticle

영어

It is hard to exclusively separate 16S rRNA from total RNAs. So the synthesized 16S rRNA has usually used or it has used partial separation method of 16S rRNAs from total RNAs using capture probe covalently linked to magnetic beads. For investigation of interaction between 16S
rRNA and several RNA binding proteins, it is needed to purify innate 16S rRNA specifically. Therefore we suggested a particular 16S rRNA separation method using a ribosomal protein, S15 from E. coli. S15 is one of proteins consitituting ribosome small subunit (30S) and binds to
16S rRNA. We overexpressed S15s in E. coli and they were binding to 16S rRNA in the cell. After cell lysis, S15s bound to 16S rRNA were separated by his-tag Ni-NTA resin. And then, we could obtain inherent 16S rRNA through the S15 removal step. This method would be helpful
to research about 16S rRNA characterization.