earticle

영어

Recently porcine islet is emerging as an alternative source for clinical islet transplantation regardless of unaddressed barriers. The purpose of this study was to utilize PEGylation (PEG-SCM/PEG-lipid 1% wt/vol) in combination with a subtheraputic dose of FK506 (0.1mg/kg, IM) in an attempt figuring out an efficient apparatus for islet transplantation. The imaging data showed that islets were well covered with PEG-lipid; furthermore, modified islets had no significant difference compared to control islets in terms of functionality and viability as evidenced by
apoptosis rate, insulin secretion, and OCR/DNA. M ore importantly, PEGylated islets revealed the PEG protective effect on islets as shown by CFSE-base assay. As in combination with FK506, the histological data suggested that engineered islets stayed intact, less infiltrated
macrophages and somehow more generated vascular compared to the control group. Taken together, our findings indicated that PEG-lipid and FK506 would be an effective regime to have islets resisted against immunological system.