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영어

The anti-aging and whitening effects of Aloe extract were studied using proteome analysis. Aloe extracts were prepared by hot water, ethanol, and supercritical fluid extraction. supercritical fluid extraction was performed at pressure of 200~350 bar and temperature of 40~70℃. The effect of extract was measured antioxidant activity [DPPH (1,1-diphenyl- 2-picryl hydrazyl) radical scavenging assay] and skin whitening activity (Tyrosinase inhibition assay). Use proteome analysis (2D-PAGE), expression level changes of the main proteins related to skin aging
inhibition and skin whitening by Aloe extracts were observed. The human fibroblasts and mouse B16F1 melanoma cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with or without extracts. Isoelectric focusing (IEF) was carried out using commercial immobiline
dry strips 24cm. SDS-PAGE was performed using 25 x 21cm, 10~12.5% polyacrylamide gel. Gels were stained with silver nitrate. Following proteome analysis could explain the mechanism and expression pattern of skin aging and whitening related proteins.