earticle

영어

Melanogenesis is a multistage process involving melanin synthesis, melanin transport and melanosome release. Melanin synthesis is stimulated by various effectors, including α-melanocyte-stimulating hormone (α-MSH), cyclic AMP (cAMP)-elevating agents (forskolin, isobutylmethylxantine, and glycyrrhizin) and ultraviolet light. In this study, the water extract from
mangosteen (garcinia mangostana) leaves was assessed with regard to its effects on melanogenesis in B16F1 melanoma cells line. The mangosteen leaves extract was shown to stimulate melanin synthesis and tyrosinase activity in a dose-dependent manner without any significant effects on cell proliferation. Formation of melanin from cultured B16F1 melanoma induced by mangosteen leaves extract treatment was estimated using spectrophotometer.
In the range of non-cytotoxic concentrations at 7.8x10-4 % ~ 6.2x10-3 % (w/v) mangosteen leaves extract strongly increased melanogenesis. The results obtained clearly shows that mangosteen leaves extract increase melanogenesis almost 2.5 folds compare to the α-MSH control group. In order to clarify the further mechanism of tyrosinase activation by mangosteen leaves extract, the level of tyrosinase expression in B16F1 cells were examine by intracellular
tyrosinase assay and tyrosinase zymography. The result showed that tyrosinase activity was markedly enhanced from mangosteen treated cells. These results suggested that mangosteen leaves extract might be one of the promising candidates in order to treat hypo pigmentation disorder and useful for self tanning cosmetic products.