원문정보
초록
영어
Aptamers are ssDNA or RNA oligonucleotides with high affinity and high specificity to their targets. SELEX (Systematic Evolution of Ligands by EXponential enrichment) is an in vitro selection and amplification technique used for selecting aptamers. There are several studies showing that Nampt, as one of adipokines, is secreted from all adipose tissue, and Nampt (visfatin/PBEF) is known to be related to various diseases such as type 2 diabetes, chronic kidney dysfunction, inflammation and cancer. Hence, aptamers binding to Nampt are expected to provide fast and sensitive diagnosis for these related diseases. A modified SELEX, FluMag-SELEX from random DNA library composed of approximately 1.2 x 1014 DNA molecules was used in this experiment. We have successfully screened ssDNA aptamers binding to the Nampt specifically.