earticle

영어

In order to enhance detected signal without an additional step in a gold nanoparticle (AuNP)-based lateral flow immunoassay (LFA), a new and simple method utilizing two AuNP- conjugates was developed. The 1st conjugate was the AuNP immobilized with an biotinylated anti-Human IL-5 antibody and blocked with bovine serum albumin (BSA), and the 2nd conjugate was the AuNP immobilized with an streptavidin and blocked with bovine serum albumin (BSA). The two conjugates were respectively encapsulated in different pads. A scheme of the LFA system is described in first figure. The size of the two conjugates was very critical in the detection sensitivity of IL-5. When 10nm for the 1st and 40nm for the 2nd were used. As compared to the conventional LFA, the detection sensitivity sucessfully increased. We could detect as low as 0.01 ng/mL IL-5 in 10 min using the dual AuNP conjugate-based LFA.