earticle

영어

Recently, QD-based fluorescence resonance energy transfer (FRET) probe as a DNA nanosensor has been studied [1]. In this experiment, PDMS-based microfluidic chips using QD-DNA probe have been fabricated for the detection of p53 gene related to genetic disease. The
PDMS microfluidic chip with a weir structure has been utilized for trapping microbeads, sample injection to wells, and fluorescence detection. Carboxylated QDs (Em: 605 nm) have been linked to microbeads of polystyrene/divinyl benzene via EDC/NHS crosslinking reaction. After
hybridization with target gene, Borate buffer with DNA intercalating dye has been loaded to the micro-channel. Fluorescence spectra have been observed from the hybridized DNA at the channel of weir structure. The FRET efficiency between QD and dye has been measured by
photoluminescence spectroscopy. This experiment shows the possibility of rapid detection of DNA via bead-QD complex on microfluidic system.