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Recently, various novel biosensors have been widely studied and developed for early diagnostics of various diseases. Dopamine is one of the large of number of neurotransmitters which plays a role as the excitement transmission in a brain cell. Development of neurotransmitter sensor using Fluorescence Resonance Energy Transfer (FRET) is expected to diagnosis or medical treatment for a disease related to the brain such as Alzheimer’s disease, Parkinsonism and High blood pressure in an aging society due to possibility for measurement of low concentration and brain pressure of the brain trouble. FRET is a physical phenomenon between two fluorophores which transfers emission energy from donor to acceptor for excitation. In this paper, FRET between carboxyl quantum dots and Alexa Fluor has been investigated and the effect of FRET efficiency depending on dopamine concentration has been studied. Quantum Dots 525 (QDs 525) as a donor has been immobilized on surface of well plate which is
modified functional group, and conjugated protein A with anti-dopamine labeled by Alexa Fluor 546 (AF 546). AF 546 is used as an acceptor. The fluorescent excitation and emission scans for two fluorophores have been obtained using photoluminescence spectroscopy (PL). The FRET efficiency was obtained from intensity ratio of the fluorescent spectra with and without
acceptor group. The dependence of FRET efficiency on dopamine concentration shows that dopamine can be detected by monitoring the FRET phenomena between two fluorescent dyes. Neurotransmitter sensor will be able to establish the system which is possible to diagnosis and medical treatment