원문정보
초록
영어
The immobilized enzyme is necessary for the development of biofuel cells which can be applied to power source of implantable devices. The carbon nanotube has been focused on applying to electrode in electrical devices because it’s nanostructure with conducting properties enables tiny
signals to be detected and transmitted, leading to the detection of target molecules at extremely low levels. Here, we explored the immobilization of glucose oxidase (GOx) on the surfaces of carboxylated multiwalled carbon tube (MWCNT) by covalent attachment using 1-ethyl-3-
(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Characterization of GOx-immobilized MWCNT was performed by FT-IR analysis and the formation of gluconic acid produced by enzyme reaction was analyzed by TLC analysis. The newly formed amide bond between GOx and MWCNT was identified by the characteristic C=O
stretching of 1634 cm-1 in FT-IR spectra. The spot of gluconic acid was observed in the reaction mixture containing immobilized GOx whereas it was not observed in the reaction mixture containing MWCNT. This result indicates that GOx was successfully immobilized on MWCNT by
covalent attachment.