원문정보
초록
영어
By the development of nanotechonology, many researchers are interested in fusion technology between NT and BT. A three-dimensional structure fabricated by nano-imprinting, called nano-pillar, has a potential to be applied in this fusion technology. We studied about fabrication of DNA
chip using nano-pillar structure for improving sensitivity. Also we fabricated different morphologies of three dimensional structures. The dense and long nano-pillar structure increased an actual immobilization amount of a probe and the sensitivity by providing high surface density. In this experiment, we immobilized a partial sequence of 5s rRNA of Legionella pnuemophila as a probe and measured fluorescence intensity with various concentration of cy3 tagged target. The maximum of fluorescence intensity of nano-pillar substrate exhibited 4.5 times higher than that of planar substrate. In addition, we examined specificity of the chip using mismatched sequence of the probe as well as sensitivity. These results showed a possibility of nano-pillar structure as a sensitive platform of biosensors.