earticle

영어

Intense requirement has grown in developing enzyme-based electrochemical biosensor possessing favorable analytical performance due to its widespread applications on biotechnology. In this regard, diverse nanostructured matrices synthesized in recent years have been widely employed as electrode due to their superior characteristics such as large surface area, controlled nano-scale structures, and inherent conductivity; however, natural enzymes are inevitably denaturated by their weakly folded structures. Thus the development of more stable artificial enzyme mimics is substantially important to improve the performance of biosensor
particularly by grafting on excellent nanostructures. Among them, Fe3O4 magnetic nanoparticles was recently reported to show intrinsic and more stable peroxidase activity than native one. Along with these lines, herein, we first demonstrate the potential of ordered m esoporous carbon, an attractive m aterial for enzyme immobilization by its unique characteristics of large pore volume to enable high loading capacity along with superior electrical conductivity, to efficiently utilize magnetic nanoparticles as next-generation peroxidase alternatives for efficient
electrochemical biosensing platform possessing excellent compatibility with other enzymatic system. To this purpose, conductive multi-catalyst system consisting of magnetic nanoparticles and glucose oxidase simultaneously entrapped in the mesopore of the carbon matrix was developed.