원문정보
초록
영어
Development of artificial tongue is important for quality control in the food and beverage industry. However, artificial tongue using inorganic synthetic materials as sensing elements, so called ‘Electronic tongues’, still have problems in terms of sensitivity and selectivity. Bioelectorinc
tongue using biological taste receptor proteins as sensing elements is expected to overcome limitations of electronic tongue. In this study, nano-vesicles containing sweet taste receptor, heterodimer composed of hTAS1R2 and hTAS1R3, derived from artificial sweet taste cells were
constructed. We created each artificial sweet taste cells stably expressing hTAS1R2 and hTAS1R3 and then the cells were transfected to express both receptor proteins. The artificial cells expressing sweet taste receptors were treated with cytochalasin B to obtain nano-vesicles budded out form the cells. The diameter of the nano-vesicles was nanometer scale which is
suitable for applying to nano-material-based sensor platform to detect specific tastant binding to receptors. This nano-vesicle production method is useful for development of receptor protein-based bioelectronic tongue. Furthermore, this work also offers useful tool for basic research on taste receptors and opens up opportunities for many industrial applications.