earticle

영어

A neural stem cell has been investigated intensively for the treatment of neurological disorders such as Alzheimer disease and PD. A neuronal stem cell is sensitive to the changes of its circumstances, which cause severe genetic mutations or cellular damages, critical for stem cell differentiation. However, the effects of environmental toxins on a neuronal stem cell has been limitedly investigated so far. In this study, we developed a neuronal stem cell-based assay to detect the effects of environmental toxins on the stem cell electrochemically. A nanopatterened peptide layer was firstly developed on an electrode surface to enhance the cellular adhesion. After cell immobilization on the substrate, cell viability was monitored electrochmeically with increasing the concentration of enivronmental toxins. The peptide-nanopatterned electrode showed stronger quasi-reversible signals from neuronal stem cells, compared to a bare gold electrode and peptide monolayer coated electrode. The electrochemical signals from neuronal stem cells on the electrode were found to decreae with increasing the concentrations of enivronmental toixns. The electrochemcial experimental data were validated by MTT assay. Our newly developed neuronal stem cell-based electrochemical system can be used to determine stem cell viability easily. Acknowledgments: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2010-0000845) and by the Nano/Bio science & Technology Program (M10536090001-05N3609-00110) of the Ministry of Education, Science and Technology (MEST) and by Seoul R&BD Program (10816).