earticle

영어

We fabricated DNA templated gold nanowire bridges on nanogap electrode arrays for electric detection of synthetic target DNA (HIV virus partial gene). The nanogap was fabricated using anisotropic silicon wet etching on SOI wafer substrate (bottom gap size was about 60nm). Target DNA of seven synthetic single-stranded DNA oligomers were designed for hybridization with capture DNAs immobilized on the nanogap gold electrode. Total 80 nm length double-stranded target DNA are connected on the two electrode nanogap forming many bridges along with the
nanogap electrode. For electric detection of the DNA bridges, DNA templated conducting gold nano wire was fabricated with positive charged gold seed particles (1.5nm) and gold seed enhancement procedure on the DNA bridges. We detected increased electric signal extremely at the HIV capture DNA immobilized nanogap electrode after fabrication of conducting gold nanobridge.