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Increased obesity characterized by excessive accumulation of adipose tissue is associated with insulin resistance and type 2 diabetes. Vaspin is an adipokine that has been isolated from visceral adipose tissue recently identified as a function of insulin-sensitizing effects. Previous studies demonstrated that its concentrations are related with insulin resistance. It means that vaspin is a useful marker for the diagnosis of type 2 diabetes. Several analytical formats for adipokines using antibody such as ELISA and western blotting have been reported because there is a high demand of an early diagnosis for diabetes and disease caused by obesity. In this
study, aptamer-based SPR biosensor, which has been known to be advantageous over other antibody-based assays, was developed to detect the vaspin levels. For this purpose, the ssDNA aptamer candidates that contain conserved sequence in random region were selected from initial
random ssDNA library pool containing 3x1014 molecules through FluMag SELEX process. It is confirmed that one of the aptamers screened shows high specificity and affinity (Kd = 0.303 ± 0.028 μM) to vaspin based on the measurement of the RU (response unit) using SPR. The minimum detection level of vaspin was found to be 25 nM by using this SPR-based sensor.