earticle

영어

DNA microarray technology enables a significant expansion of the molecular identification and characterization of multiple species in food safety, medical, agricultural and public health. In this study, we focused on development of species identification DNA microarray chips for pathogens. Three food-borne pathogens were selected for a random genomic DNA fragment-based chip,
Staphylococcus aureus, Salmonella enterica and Bacillus cereus. At first we followed probe development protocols, the strategy of a random genomic DNA fragment, and constructed the proto-type DNA microarrays which have about 50 probes of each strain. It was validated and optimized for the detection of significant strains. To discriminate significant probes in the proto-type DNA microarray, we have successfully tested seven food-borne pathogens such as,
Escherichia coli O157, Enterobacter sakazakii, Listeria monocytogenes, Yersinia enterocolitica, Alicyclobacillus acidocaldarius, Alicyclobacillus acidoterrestris, and Alicyclobacillus cycloheptanicus including E. coli as a non-pathogen. Finally, we have completed the probe contents after removing the probes cross-talked with other non-specific species, and successfully developed a specific DNA microarray which can be used to detect 3 specific target pathogens and discriminate non-specific pathogens.