earticle

영어

L-Homocysteine (L-Hcy) is a thiol containing amino acid that is formed as an intermediate when methionine is converted to cysteine. L-Hcy is known as an important independent risk factor of cardiovascular disease. The early diagnosis and treatment of hyperhomocysteinemia is critical for public health care and metabolic disease patients including homocystinuria. It has been used for the determination of total L-Hcy concentration by several methods, such as HPLC, stable isotope dilution, and enzyme immunoassay. However, they are all time-consuming and require highly skilled technical staffs. Here we developed new L-Hcy assay method by using a hybridoma producing monoclonal antibodies that specifically recognize L-Hcy. Monoclonal antibodies specific to L-Hcy were produced using L-Hcy coupled to BSA with glutaraldehyde as an antigen. The enlarged lymph nodes from rats injected via hind footpads with an emulsion of antigen and Freund’s adjuvant were used for cell fusion. Hybridoma clones secreting the monoclonal antibodies were selected by ELISA.