earticle

영어

Plasma L-homocysteine (L-Hcy) is known to be a risk factor of various diseases. The measurement of plasma L-Hcy concentration is important for the prediction of several clinical conditions including homocystinuria, atherosclerosis, and thrombophilia. Accordingly, the interest in Hcy determinations has increased in research laboratories and companies.
Several companies are already producing the kits for the detection of L-Hcy concentration. However, those kits need enzymatic conversion process of Hcy to S-adenosyl-L-homocysteine. We have generated monoclonal rat anti-Hcy antibody that specifically recognize the Hcy, so it will give big merits to simplify the detection method by elimination of reduction and conversion process. We could assay the localized endogenous L-Hcy by immunofluorescence in calf pulmonary artery endothelial (CPAE) cell for the first time by the direct monitoring of Hcy in living cells using our developed antibody. And competitive ELISA data suggest that the sensitivity for the detection of free L-Hcy by developed anti-Hcy monoclonal antibody is less than 100 nM level which is about ten times higher than previous detection kit.