earticle

영어

Mucosal vaccination has been considered as an attractive immunization principle based on the consideration that mucosal surfaces are vulnerable for invasion by pathogen. In that sense, transgenic plant is noticed as promising carrier offering attractive option for production and delivery of mucosal vaccine. Classical swine fever (CSF) is a highly contagious viral disease of
pigs and results in devastating financial losses. Because CSFV E2 glycoprotein mediates the viral attachment and penetration to susceptible cells, it is considered as a target for inducing protective immunity. In this study, in order to develop the vaccine materials for mucosal immunization against the CSFV infection, fully codon optimized E2 was introduced into the rice and expressed under the control of RAmy3D sugar starvation inducible promoter. The proteins expressed in the transgenic rice cultured cells effectively induced immune responses after intraperitoneal and oral administration. In addition, E2 conjugated with M cell targeting ligand, Co1, and we are currently analyzing immune responses induced by oral administration of E2/Co1-expressing rice calli (This work was supported by the grant from MIFAFF)