earticle

영어

Recently, the rice α-amylase 3D promoter system activated under sucrosestarved conditions has emerged as an alternative system for the production of recombinant proteins. However, the influence of N-linked glycan structures related to sugar starvation conditions has not yet been investigated. The N-glycan patterns obtained from glycoproteins grown in growth medium with sucrose (+S) were different from those obtained under sucrose starvation (-S). The mass spectra of +S glycans exhibited eleven N-glycan patterns: MMXF3, GnMX, GnMXF3, GnGnX, AMXF3, GnGnXF3, AGnXF3, (FA)GnXF3, AAXF3, (FA)(FA)X, and (FA)(FA)XF3. The five N-glycans that disappeared under -S conditions were GnMX, GnGnX, AAXF3, (FA)(FA)X, and (FA)(FA)XF3. The N-glycan pattern of hGM-CSF derived from rice suspension cell culture was similar to that of -S condition, and all N-glycans contained plant-specific β1,2-xyolse, core α1,3-fucose, and some Lewis a epitopes. High-mannose type N-glycans were not detected under either condition (-S or +S) among the secreted glycoproteins. (This Study was supported by Technology Development Program of MIFAFF, and also by a grant from the Ministry of Knowledge Economy)