earticle

영어

A rice cell suspension culture system with the RAmy3D promoter, which is induced by sucrose starvation, has been previously utilized to produce large quantities of recombinant proteins. However, using rice as production system for therapeutic proteins requires modification of their
N-glycosylation pattern because of the immunogenicity of plant-specific sugar residues. In this study, for generating glyco-engineered rice as production host for therapeutic glycoproteins, an intron-containing selfcomplementary hairpin RNA-mediated post transcriptional gene silencing
was used to obtain a targeted down-regulation of the endogenous glycosyltransferase genes in rice cell suspension cultures. N-linked glycans from the generated RNAi lines were identified and their structures were compared with those isolated from the wild type suspension cells.
The Δ3FT/XT glyco-engineered line with significantly reduced xylosylated and/or core fucosylated glycan structures. (This Study was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries and also by a grant from the Ministry of Knowledge Economy)