원문정보
초록
영어
Quality control of glycan profiles is a critical issue in the biogical and pharmaceutical industry. Recently, the glycan profile method which combined with Girard's reagent T (GT) and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was
exploited for qualitative and quantitative analysis of glycans. In this study, we present a rapid and high-throughput N-glycan release, purification, methyl esterification and GT derivatization method using 96-well plate based procedures. The N-glycans were released from model glycoprotein using a polyvinylidene fluoride membrane filter plate, than subsequently loaded onto porous graphitic carbon containing 96-well plate to remove the salts. The purified N-glycans were neutralazated by methyl esterification using 96-well plate, than GT derivatizated for generate a permanent cationic charge to the reducing end of neutral glycan. finally, the N-glycans were characterized by qualitative and quantitative analysis using matrixassisted
laser desorption/ionization time-of-flight mass spectrometry. It is believed that the established 96-well plate platform described here may contribute to the rapid screening of potential N-glycan biomarkers.