원문정보
초록
영어
The development of Porcine Cholera Marker Vaccine is keenly required, because economical losses were very big on pig breeding market in the world. The baculovirus expression vector system was used for the production of heterologous recombinant proteins as a powerful expression vector system. Undefined fetal bovine serum has several defects following downstream processing, contamination and variation in commonly using insect cell cultures. Therefore, the marker vaccine is also required to be produced by serum free media. It is possible for commercially produced several SFM (Sf 900 II, Sf900 III, Ex-Cell 405, Ex-Cell 420, Ex-Cell Titer High, Express- Five, SFX-insect, Serum Free and Protein Free insect medium-1) to achieve high cell density of Sf9 cells and high yield of recombinant baculovirus. The production of porcine cholera marker vaccine was investigated by infection of recombinant baculovirus into Sf9 cells in various SFM. An optimum SFM for the production of marker vaccine were searched. The result of the study showed that two SFM (sf900 III and ex-cell 420) yielded similar productivity to 10% serum supplemented TNM-FH medium. Since Sf900 III showed slightly higher
yield than Ex-Cell 420, SF-900 III was selected for optimum serum free medium for the production of porcine cholera marker vaccine resulting recombinant baculovirus titer of 4.25x107 pfu/cells at 0.1MOI.