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초록
영어
Recently, as a result of drastic progress in nanotechnologies, the use of nanoparticles and quantum dots has significantly surged to various applications from scientific- to industrial field. In particular, the nanoparticles has expanded to be used in our daily life such as garment, tooth brush, and cosmetics. However, toxicity by nanoparicles has been controversially argued and, to date, great efforts have been devoted to prove the toxicity of nanoparticles. Titanium dioxide (TiO2) nanoparitcles have physical properties diffusing and reflecting UV radiation; due to these properties, cosmetics of sun-cream contain TiO2 nanoparticles to block UV radiation. In this work, the toxicity of silicagel-coated TiO2 nanoparicles on skin- and lung cell was investigated
using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium (MTS) assay- and confocal microscopy for live & dead of cells. The skin- and lung-cell were parcelled out from Korean Cell Line Bank. In addition, E. coli growth curve of TiO2-treated cells was measured. We observed that TiO2 nanoparticles impedes the cell growth (using MTS), and dead cells increase depending on the concentration of TiO2 nanoparticles using confocal microscopy. It was also confirmed that E. coli growth curve is influenced by the concentration of TiO2 nanoparticles. Based on the results mentioned above, we discuss the toxicity and the safety of TiO2 nanoparicles for the human cells.