earticle

영어

Primary culture of hepatocytes is an in vitro model widely used to investigate various aspects of liver physiology and pathlogy. Type I rat tail collagen is usually used as a coated material for hepatocyte culture. It promotes attachment and growth of hepatocytes. Here, we performed the potential use of a recombinant mussel adhesive protein (MAP), conjugated with type I collagen
derived mimetic peptide as a cell proliferation and differentiation in hepatocyte. Analyses of cell morphology by light and scanning electron microscopy demonstrate that a culture system, in which key components of the extracellular matrix (ECM) have been optimized, can support a differentiated phenotype of primary rat hepatocytes. Furthermore, the results indicate that the
MAPTrixTM-C (Collagen I mimetic) is similar morphology compare with other ECM systems. Using MAPTrixTM-C (Collagen I mimetic), differentiated cell morphology can be maintained for at least two weeks. Tight clusters of spherical cells with a lumen appear within the first day of culture in the MAPTrixTM-C (Collagen I mimetic). Cells will not spread, but remain in
clusters throughout the entire culture period of two weeks. Cellular functions typical of differentiated hepatocytes are maintained for a at least two weeks as shown by continued expression of cytochrome P450, a hemoprotein involved in the oxidative metabolism of various compounds, an important function of the liver. Therefore, the MAPTrixTM-C (Collagen I mimetic) is suitable for use as a cell-adhesion material in hepatocyte culture.