earticle

영어

Adhesion of cells on surface is a basic and important concept in the fields of neural cell culture and morphogenesis of the nervous system. An appropriate material for cell adhesion generally leads to improved differentiation of neuronal cells. Here, we investigated the potential use of a recombinant mussel adhesive protein (MAP), conjugated with laminin derived mimetic peptide as a cell-adhesion material, cost-effective and high-level production in Escherichia coli. Extracellular matrix (ECM) affect process outgrowth, branching of neurites, and profile of neural cells. MAPTrixTM ECM-laminin coatings support cell with the similar diverse morphology compare with other ECMs. ECMs affect adhesion, MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin coatings have greater adhesion compare with
poly-l-lysine. ECMs affect expression and growth of axons and dendrites. Greatest number of dendrites and axons are seen on MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin coatings. ECMs affect polymerization of actin filaments. High levels of F-actin are produced on MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin coatings. ECMs affect expression of the glutamate neurotransmitter
receptor. The highest levels of GluR1 are found on MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin. Therefore, the MAPTrixTM ECM-laminin is suitable for use as a cell-adhesion material in neural cell culture.