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포스터 발표 : 동물 및 식물세포공학

Purification and Characterization of Trypsin from Transgenic Rice Cell Suspension Culture

초록

영어

Bovine trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is widely used for commercial purposes to digest or process other proteins. Plant production system of recombinant proteins offers a way to replace animal-derived proteins with a safe and economical alternative. Recombinant bovine trypsin (rbtrypsin) was produced in transgenic rice cell suspension culture under the control of rice α-amylase 3D promoter (RAmy3D) which is highly expressed during sugar starvation. The
expression level of rbtrypsin was 20mg/L. It was purified from transgenic rice cell suspension culture medium by hydrophobic interaction chromatography and size exclusion chromatography. From 1L flask culture, 0.55mg pure active rbtrypsin was obtained. The enzyme activity of purified rbtrypsin was generally characterized by zymogram. These results demonstrate a possibility to use the rbtrypsin derived from rice cell suspension culture for commercial purposes. (This Study was supported by a grant from the Next Generation New Technology Development Project of the Ministry of Knowledge Economy).

저자정보

  • Eun-Sun JEUNG Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Yun Ji SHIN Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Hyo Boon KIM Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Young Eun YOO Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Min Woo NAM Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Hong-Soo DOO Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Moon-Sik YANG Division of Biological Sciences, Chonbuk National University, Jeonju 561-756, KOREA.
  • Tea-Ho KWON Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.

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