원문정보
초록
영어
Bovine trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is widely used for commercial purposes to digest or process other proteins. Plant production system of recombinant proteins offers a way to replace animal-derived proteins with a safe and economical alternative. Recombinant bovine trypsin (rbtrypsin) was produced in transgenic rice cell suspension culture under the control of rice α-amylase 3D promoter (RAmy3D) which is highly expressed during sugar starvation. The
expression level of rbtrypsin was 20mg/L. It was purified from transgenic rice cell suspension culture medium by hydrophobic interaction chromatography and size exclusion chromatography. From 1L flask culture, 0.55mg pure active rbtrypsin was obtained. The enzyme activity of purified rbtrypsin was generally characterized by zymogram. These results demonstrate a possibility to use the rbtrypsin derived from rice cell suspension culture for commercial purposes. (This Study was supported by a grant from the Next Generation New Technology Development Project of the Ministry of Knowledge Economy).