원문정보
초록
영어
We compared characterization of the cells by different isolation methods, mechanical dissociation and collagenase digestion (type I or IV). Although the recovery number was different from the groups, cellular morphology and growth rate showed a similar pattern. The expression for cellular markers was analyzed by imunocytochemistry and flow-cytometry. The result showed that Collagen I and Thy-1 is positive expression and Collagen IV, vWF, and PECAM is negative expression. Especially, positive expression of TRA-1-60 showed only mechanical dissociation. Finally, we confirmed the adipogenic differentiation abilities of the both cells. While the cells by mechanical dissociation formed mature adipocyte after 6 weeks in induction medium,
collagenase isolated cells were revealed adipogenic cell colonies after 3 weeks under the same conditions. In summary, isolated cells from different method in this study showed a similar morphology and growth yield. The cells from collagenase digestion exhibited higher rates of differentiation into adipocytes. The relation between marker expression and differentiation
ability are additionally investigating to identify the UCT derived progenitor cells.