earticle

영어

Carbon dioxide capture technologies have the potential to become an important climate change mitigation option through sequestration of gaseous CO2. A new concept for CO2 capture involves use of carbonic anhydrase (CA) that catalyzes the reversible hydration of CO2 to HCO3 -
and H+. Also, CA is important in biological systems because the uncatalysed interconversion between CO2 and HCO3 - is slow. In this study, we developed multiple localization-controlled biocatalyst which can express CA efficiently. At first, we built up vector system which can
express CA of synechocystis PCC6803&Escherichia coli K12 at cytoplasm and periplasm and excuted cloning. (expressing CA of synechocystis PCC6803 at cytoplasm, periplasm and CA of E.coli K12 at cytoplasm, periplasm) Next, cloned recombinant vector was transformed into
BL21(DE3). Expressed protein(CA) was checked as western blotting. At the end, we could confirm CA expression at different vector system.