earticle

영어

5.6 Million ton/year of rice straw is produced in Korea. If this amount of rice straw is used for lignocellulosic biothanol production, it can replace more than 10% of the whole transport gasoline use in Korea. In this study, the ethanol conversion rate was evaluated by simultaneous saccharification and fermentation (SSF) for the development of bioethanol production techniques from dilute-acid pretreated rice straw biomass. From analysis of NREL standard quantification
method of cellulosic biomass, Rice straw consisted of 40~42% of cellulose, 23~25% of hemicellulose, 20~22% of lignin and 10-12% of ash, and 2% of etc. The sample was pretreated with 1% H2SO4 solution at 160℃ for 5 minutes using cylinder type reactors. Pretreated sample contained of 39% of glucan. Alcohol fermentation was performed according to NREL standard protocols of SSF. For the enzymatic reaction, cellulase complex and β-glucosidase were added as 30FPU/g and 30CBU/g respectively. Before the fermentation seed culture was carried out with Saccharomyces cerevisiae KACC30008 at 30℃ for 24 hours. Culture media consisted of 5% of glucose, 1% of yeast extract and 2% of peptone. SSF fermentation was carried out for 72 hours and ethanol yields of α-cellulase, dilute-acid pretreated rice straw and hot water pretreated rice straw were 92.3%, 81.2% and 25.7%, respectively. In conclusion, it was confirmed that one ton of
rice straw produced 214L of ethanol. which was 73.3% of theoretical yield.