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논문검색

포스터 발표 : 해양생물공학

Improvement of M3 Alginate Lyase Activity by Homology Modeling-derived Mutations

원문정보

Yoo jung PARK, Hee Sook KIM, Eun Yeol LEE

한국생물공학회 한국생물공학회 학술대회 2010 춘계학술대회 및 국제심포지움 2010.04 pp.178-178
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Poly-guluronate specific lyase from Streptomyces sp. strain M3 from marine algae was cloned and expressed in E. coli BL21(DE3), previously. Partialy purified M3 alginate lyase was showed temperature stability at 50-60℃, whereas ALG-5 lyase which has 7 different aminoacid residues
showed temperature stability at 30-40℃. So, we carried out homology modeling and compared the energy level between M3, 1UAI and ALG-5 alginate lyases using Prosa 2003 and AutoDock Vina program for improvement of enzyme activity or stability by site directed mutagenesis.
Homology modeling-derived mutagenesis showed the improving effects on alginate degrading activity. Acknowledgement : This work was financially supported by the Ministry of Knowledge Economy(MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.

키워드

저자정보

  • Yoo jung PARK Dept. of Biotechnology, Kyungsung University, Busan 608-736.
  • Hee Sook KIM Dept. of Biotechnology, Kyungsung University, Busan 608-736.
  • Eun Yeol LEE Dept. of Chemical Engineering, Kyung Hee University, 446-701.

참고문헌

자료제공 : 네이버학술정보

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