earticle

영어

Marine microalgae, Spirulina maxima was extracted under two different conditions: water at 100℃(W-100), water at 60℃ followed by ultrasonification(UW-60). It was found that the extracts from ultrasonic process at low temperature had better anticancer activity than that from W-100. The two major molecular weights were measured as 2105 daltons and 2146 daltons from the MALDI-TOF of W-100. But, the extract at 60kHz at 60°C (UW-60) had smaller molecules than that at W-100, ranging from 387 to 490 daltons, which tells that ultrasonification process effectively digested the proteins more than the simple water extraction at 100°C. In comparison with HPSEC
chromatograms of UW-60 and W-100, W-100 showed four small polysaccharide at 56, 63, 78 and 84 minutes in the HPSEC chromatogram, indicating the occurrence of significant degradation of polysaccharide at 100°C. Under water extract at 60oC with 60kHz frequency, polysaccharides were mostly eluted in a high and narrow peak at 88 minutes. Interestingly, the ultrasonification process did not affect polysaccharide degradation, but digestion of proteins in microalgae. These study results showed that extraction using 60kHz frequency at 60°C with water solvent can effectively extract intact biologically active polysaccharides and not proteins.