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Reactive oxygen species (ROS) have been shown to damage tissues. Thus, ROS are associated with tissue damage and the prime contributing factors for inflammation, hypertension, diabetes, aging, cancer and arteriosclerosis. Some mushrooms, including Phellinus linteus and
Inonotus hispidus, commonly produce a bundle of yellow antioxidant pigments that are composed of hispidin derivatives and polyphenols. Hispidin, metabolite of Phellinus linteus, is a potent antioxidant and inhibitor of protein kinase C (PKC). In this study, it was investigated
hispidin could protect MIN6N pancreatic β-cells from hydrogen peroxide. Treatment of MIN6N β-cells with 0.5mM hydrogen peroxide for 4 hours could cause a significant cell viability loss and an increase in apoptotic cells. Pre-treatment of MIN6N β-cells with hispidin for 24 hours reduced cell viability loss and decrease in apoptotic cells. In addition, 70μM hispidin significantly scavenged the intracellular ROS and inhibited apoptosis induced by hydrogen peroxide. These results suggest that hispidin reduces oxidative stress of MIN6N β-cells by hydrogen peroxide. Hispidin may be effective in preventing MIN6N β-cells from the toxic action of reactive oxygen species in diabetes.