earticle

영어

The aim of the present study was to determine inhibitory effects of COSs on in vitro allergic reactions in rat basophilic RBL-2H3 cells. Our results revealed that COSs were effective in suppressing the releases of histamine and β-hexosaminidase from RBL-2H3 cells stimulated with
Ionophore A23187 or immunoglobulin E (IgE)-antigen complexes. The extent of inhibition of COSs was accompanied by intracellular Ca2+ levels. In addition, COSs inhibited the A23187 plus PMA -induced increases in TNF-α, IL-1β, IL-4, and IL-6 production and expression. Moreover, RT-PCR and Western blot analysis revealed that COSs reduced the regulation of antigen-stimulated FcεRI, the high affinity IgE receptor. COS with molecular weight of 1-3 kDa was the most effective inhibitor in all assays. Taken together, the in vitro anti-allergic effects of COSs suggest possible therapeutic applications for this agent in allergic diseases through the inhibition of degranulation dependent on the decrease of intracellular Ca2+ levels, inflammatory cytokines and the high affinity IgE receptor expression in RBL-2H3 cells.