원문정보
초록
영어
Human basophilic KU812F cells play an important role in allergic reactions. In the present study, the effect of solvent fractions from Ishige okamurae on allergic response was examined by measuring histamine release, cytokine production, and FcεRI expression in human basophilic
KU812F cells. Among solvent fractions from I. okamurae, more than 70% of the degranulation of KU812F cells was inhibited by n-hexane and EtOAc (Ethyl acetate) fractions. In addition, n-hexane and EtOAc fractions inhibited the increased IL-4 and IL-13 production and expression. RT-PCR and western blot analysis revealed that n-hexane and EtOAc fractions reduced the regulation of FcεRI α, β and γ chains. Therefore, I. okamurae may exert its anti-allergic activity through negative-regulation of FcεRI expression, inhibiting IL-4 and IL-13 productions and expressions, and decreasing histamine release. Furthermore, we isolated and quantified the polyunsaturated fatty acids from EtOAc fraction with the highest anti-allergy activity as candidate components for anti-allergic activity. These results suggest that I. okamurae exert potent anti-allergic activity, and it could be effectively used in production of anti-allergic pharmaceuticals and cosmaceuticals.