원문정보
초록
영어
Clostridium beijerinckii is a gram-positive, spore-forming, obligate anaerobic bacterium, which is able to produce solvents acetone, butanol and ethanol using various sugars as a carbon source. A gene expression reporter system (pCBEst-MTL) for C. beijerinckii NCIMB 8052 was developed by using the esterase gene from Clostridium as a reporter gene. In order to test the reporter system, promoters of key metabolic pathway genes, ptb (coding for phosphotransbutyrylase) and thl (coding for thiolase) from C. beijerinckii NCIMB 8052, were cloned upstream of the reporter gene in pCBEst-MTL. Detection of esterase activity performed with recombinant strains demonstrated that each reporter gene expression system produced functional esterase in C. beijerinckii.