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Metabolic Engineering of Clostridium acetobutylicum ATCC 824 for Enhanced Butanol Production

원문정보

Jongho YI, Yu-Sin JANG, Jin Young LEE, Joungmin LEE, Sang Yup LEE

한국생물공학회 한국생물공학회 학술대회 2010 춘계학술대회 및 국제심포지움 2010.04 pp.154-154
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초록

영어

In recent years, interest in butanol as potential fuel has grown up due to its advantages over ethanol such as high energy density, phase stability on blending with other fuels, and non-corrosiveness. Clostridium acetobutylicum ATCC 824 is the most frequently used organism for butanol production by ABE fermentation. The present work was carried out to develop a strain of Clostridium acetobutylicum with improved butanol production capabilities. To achieve this objective, a gene knockout system was constructed by using an L1.LtrB group II intron from Lactococcus lactis. The transcription of the L1.LtrB intron on pCACYS3 was controlled by a thl promoter. Based on this knockout system, mutants on acetic and butyric acids pathways have been constructed from C. acetobutylicum ATCC 824 and characterized. [This work was
supported by the Korea–Australia Collaborative Research Project on the Development of Sucrose-Based Bioprocess Platform (#10030795) from the Korean Ministry of Knowledge Economy. Further support by GS Caltex, BioFuelChem, Microsoft, and the WCU Program (R32-2008-000-10142-0) of the MEST are appreciated.].

키워드

저자정보

  • Jongho YI Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury.
  • Yu-Sin JANG Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury.
  • Jin Young LEE Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury.
  • Joungmin LEE Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, and Department of BioSystems and Bioinformatics Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Korea.
  • Sang Yup LEE Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, and Department of BioSystems and Bioinformatics Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Korea.

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