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Effect of coexpression of protein folding accessory proteins on the production of hepatitis B virus surface antigen in recombinant Saccharomyces cerevisiae

원문정보

Yi-Hyun BAE, Young-Kyoung PARK, Jin-Ah ROH, So-Yeon SHIN, Hyung-Kweon LIM, Yong-Cheol PARK, Jin-Ho SEO

한국생물공학회 한국생물공학회 학술대회 2010 춘계학술대회 및 국제심포지움 2010.04 pp.152-152
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초록

영어

Hepatitis B virus causes the acute and chronic liver diseases. The gene coding for the S domain of hepatitis B virus surface antigen (sHBsAg) fused with the mating factor α (MFα) signal sequence was introduced by the chromosomal δ-integration to make S. cerevisiae 2805/pδMFα_sHBsAg strain. The sHBsAg gene was expressed under the control of the galactoseinducible GAL1 promoter. In this study, protein folding accessory proteins such as PDI1, SEC23, TRX2 and SCJ1 were coexpressed individually in recombinant S. cerevisiae 2805/pδMFα_sHBsAg to enhance the production of sHBsAg. Their coexpression led to a 2-fold increase each in the expression level of sHBsAg, compared to that of the control strain. The combinatorial expression of PDI1 and SEC23 gave a synergistic effect on the sHBsAg titer
and productivity. Also KEX2 protease was coexpressed to obtain intact sHBsAg. In a glucose-limited fed-batch fermentation with YPDG medium, 15.2 g/L of dry cell mass and 5.81 mg/g cell of specific sHBsAg content were obtained in recombinant S. cerevisiae 2805/ pδMFα_sHBsAg +pPDI1+ pSEC23+pKEX2, which were approximately 11 and 12 times higher than those of the control strain, respectively.

키워드

저자정보

  • Yi-Hyun BAE Dept. of Agricultural Biotechnology, Seoul National University, Seoul, 151-742.
  • Young-Kyoung PARK Dept. of Agricultural Biotechnology, Seoul National University, Seoul, 151-742.
  • Jin-Ah ROH Dept. of Agricultural Biotechnology, Seoul National University, Seoul, 151-742.
  • So-Yeon SHIN Dept. of Agricultural Biotechnology, Seoul National University, Seoul, 151-742.
  • Hyung-Kweon LIM Mogam Biotechnology Research Institute, Yongin, Kyunggi.
  • Yong-Cheol PARK Dept. of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul, Korea.
  • Jin-Ho SEO Dept. of Agricultural Biotechnology, Seoul National University, Seoul, 151-742.

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자료제공 : 네이버학술정보

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