원문정보
초록
영어
3-hydroxypropionic acid (3-HP) is expected to build up huge market in biomass derived valuable platform chemicals recently and its commercial mass production will led to bio-refinery industrial development. In glycerol metabolism of bacteria, 3-HP is generally known as converting from
3-hydroxypropionaldehyde (3-HPA) by aldehyde dehydrogenase. To find out the appropriate enzyme for achieving the effective catalysis, 32 kinds of gene encoding each of the dehydrogenase from Lactobacillus reuteri were cloned with dhaT which encoding 1,3-propandiol oxidoreductase, and expressed in Klebsiella pneumoniae AK strain (1). Among 32 recombinants,
a half of them illustrated enhancement aspects comparing with the control while eight showed lower 3-HP production. The highest 3-HP production was investigated in the recombinant strain which containing a gene encoding propanediol utilization protein. The enzyme will be useful for
improvement of the 3-HP production.