earticle

영어

Enzymes catalyze industrially important reactions in organic solvents. But their activity in anhydrous media is extremely low compared with its activity in water media. It is due to decrease of enzyme motion in the absence of lubricant water followed by enzyme flexibility decrease. Enzyme flexibility has relation to enzyme activity because enzyme does flexible motion for catalysis. Thus, enzyme flexibility modulation can lead enzyme activity enhancement. In this study, Candida antarctica lipase B(CalB) was selected as a model enzyme for mutations to change enzyme flexibility. From the analysis using spring model, it was detected that forced and twisted regions of CalB were located in residues surrounding active site. The edges of these helices were selected as target sites aiming at increasing flexibility. The details will be presented and discussed.