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포스터 발표 : 효소공학

A novel enzyme support derived from cellulose and chitosan

원문정보

Seulji AN, Min Hoo KIM, Young-Hoo KIM, Hyung Joo KIM, Keehoon WON, Sang Hyun LEE

한국생물공학회 한국생물공학회 학술대회 2010 춘계학술대회 및 국제심포지움 2010.04 pp.145-145
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

A novel enzyme support derived from cellulose and chitosan was prepared under an environment-friendly preparation processes using ionic liquids. Cellulose and chitosan were codissolved in [Emim] [acetate]. The obtained clear and viscous solution was introduced dropwise into water bath using a syringe needle. Then the hydrogel beads were collected and extensively washed with deionized water. Prepared cellulose-chitsoan composite bead was treated with glutaraldehyde and then used as the support to immobilize formate dehydrogenase. Formate dehydrogenase was successfully cross-linked on the surface of cellulose-chitosan bead. Immobilized enzyme was easily reused and remained activity after 5 times reuse was over 95% of initial activity. Thermostability and pH stability of formate dehydrogenase immobilized on cellulose-chitosan bead were also highly enhanced. Half-life times of immobilized enzymes were 1.8 and 7.1 times increased at 52℃ and pH 4.3, respectively.

키워드

저자정보

  • Seulji AN Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701.
  • Min Hoo KIM Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701.
  • Young-Hoo KIM Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701.
  • Hyung Joo KIM Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701.
  • Keehoon WON Dept. of Chemical and Biochemical Engineeringm, Dongguk University, Seoul, 100-715.
  • Sang Hyun LEE Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701.

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자료제공 : 네이버학술정보

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