earticle

영어

Alginate lyase from a novel marine microorganism, Streptomyces sp. ALG-5, was expressed in the presence of various molecular chaperones in Escheichia coli BL21(DE3). The hydrolytic degradation activity toward 0.4 %(w/v) alginate solution was two times enhanced when the alginate lyase gene was expressed in pColdI plasmid than pET-21b(+) at low temperature, 15oC. The reaction condition of alginate lyase-catalyzed hydrolysis of alginate was optimized. The purified enzyme was immobilized onto the Fe3O4/silica/NiO magnetic nanoparticles efficiently, and re-used five times with retention of more than 50% of initial activity in repeated batch operations.