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영어
In order to enhance the activity of the recombinant epoxide hydrolase of Caulobacter crescentus(CcEH), the EH gene was inserted to pColdI vector, pET21b(+) vector and pET28b vector, then expressed in the presence of various molecular chaperones and foldases. The specific
activity of CcEH was the highest when the gene was expressed with pTf16 plasmid containing tig gene. We carried out enantioconvergent hydrolysis of racemic 4-chlorostyrene oxide and styrene oxide for the preparation of enantiopure (R)-4-chrolopheny-1,2-enthanediol and (R)-phenyl-1,2-ethanediol. The styrene oxide and 4-chlorostyrene oxide were analyzed by a chiral GC, and the corresponding diols were analyzed by a chiral HPLC. Chiral (R)-4-chrolopheny-1,2-enthanediol and (R)-phenyl-1,2-ethanediol with enantiopurity more than 98% was obtained with yield greater than 50%.