earticle

영어

The oleandomycin glycosyltransferase (OleD) gene from streptomyces antibioticus was cloned by PCR, and then inserted to pColdI and pET-21b(+) vector, respectively. The recombinant OleD gene was functionally expressed in the presence of various molecular chaperones and foldases to prevent protein aggregations in Escherichia coli. In order to analyze their abilities for glycosylation, the OleD crude cell extracts were reacted with 4-methylumbelliferone and the reaction progresses were measured by fluorescence spectrometer. We investigated the substrate
specificity of OleD towards various flavonoids such as apigenin, chrysin, daidzein, genistein, kaempferol, luteolin, naringenin, resveratrol, quercetin and etc. with uridinediphosphate-activated glucose. The chemical structures of the flavonoids glucosides were determined, and the catalytic mode of the OleD was discussed. Acknowledgement: This work was supported
by the National Research Foundation of Korea(NRF) grant funded by the Korea government (MEST) (No. 2009-0088439). This work was supported by the Korea Student Aid Foundation (KOSAF) grant funded by the Korea government (MEST) (No. S2-2009-000-00624-1).